Fungal Biotransformation of Chlordiazepoxide and Evaluation of Type and Kinetics of Fungal Enzyme

To produce an active metabolite of Chlordiazepoxide by fungal biotransformation in easy and economic way also to develop microbial models for drug metabolism studies. is metabolized the liver CYP3A4 forms major N-desmethyl chlordiazepoxide. The focus study was explore ability six distinct fungi biotransform its metabolites. Induction, Inhibition kinetic studies were conducted find out type capability enzyme involved biotransformation. screening performed fermentation protocol designed with two controls (culture control control) one sample. Extract samples reconstituted analysed using HPLC. similarly maintaining respective inducer (Carbamazepine) inhibitor (Fluoxetine), further Km Vmax Chlordiazepoxide. Among organisms Aspergillus ochreus has shown extra peak at 6.9 min. HPLC when compared indicated formation metabolite. thus formed identified, isolated structure confirmed mass spectrometry NMR spectroscopy as Nor-chlordiazepoxide. During inhibition induction studies, it found that quantity increased decreased inhibitor. 1.928 µg/ml 0.1802 µg/ml/hr respectively. like followed Michaelis-Menten kinetics.